Journal: BMC Women's Health
Article Title: Increased capsaicin receptor TRPV1 in skin nerve fibres and related vanilloid receptors TRPV3 and TRPV4 in keratinocytes in human breast pain
doi: 10.1186/1472-6874-5-2
Figure Lengend Snippet: TRPV3-immunoreactivity in breast skin . TOP PANELS: TRPV3-immunoreactive keratinocytes mostly in basal layer and graded from grade 0/negative (top left) to grade 3/strong staining (bottom right). Scale bar = 100 μm BOTTOM PANEL: Scattergram showing grading assessment and a significant increase (*P < 0.05) of TRPV3 immunoreactivity in patients with pain.
Article Snippet: After a further wash in PBS the tissue sections were incubated overnight with affinity purified antibodies to TRPV1 (polyclonal rabbit anti-TRPV1; GlaxoSmithKline, Harlow, UK; 1/5000; 1/10,000), TRPV3 (polyclonal rabbit anti-TRPV3; GlaxoSmithKline, Harlow, UK; 1/1000), TRPV4 (polyclonal rabbit anti-TRPV4; GlaxoSmithKline, Harlow, UK; 1/250; 1/1000), recombinant human NGF (polyclonal rabbit anti-NGF; Genentech, San Francisco, USA; 1/4000) or marker of large and some small calibre nerve fibres (mixed mouse monoclonal antibodies to neurofilaments 200 kD, 70 kD and 57 kD; DAKO cytomation Cambs., UK, 1/50,000; Novocastra, Newcastle upon Tyne, UK, 1/500).
Techniques: Staining